Extraction and purification of total DNA from soil for PCR identification of microorganism composition

DEAGHILEVA, Angela

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dc.contributor.author	DEAGHILEVA, Angela
dc.date.accessioned	2022-11-15T11:20:42Z
dc.date.available	2022-11-15T11:20:42Z
dc.date.issued	2022
dc.identifier.citation	DEAGHILEVA, Angela. Extraction and purification of total DNA from soil for PCR identification of microorganism composition. In: Modern Technologies, in the Food Industry – 2022: proc. of the Intern. Conf., MTFI – 2022, 20-22 October 2022. Chişinău: Tehnica-UTM, 2022, p. 77. ISBN 978-9975-45-851-1.	en_US
dc.identifier.isbn	978-9975-45-851-1
dc.identifier.uri	http://repository.utm.md/handle/5014/21682
dc.description.abstract	Monitoring the composition of the soil microbiome is an important aspect of successful vineyard management. Microorganisms involved in the production of wine during the fermentation process affect its metabolite profiles and, ultimately, quality. Firstly, for successful identification of the species composition of soil microflora, using PCR analysis, high quality DNA free from inhibitors is required. Secondly, representative amount of microorganisms’ DNA must be in the sample. This paper outlines the combination of several methods of DNA extraction and purification from the soil, suitable for PCR analysis.	en_US
dc.language.iso	en	en_US
dc.publisher	Universitatea Tehnică a Moldovei	en_US
dc.rights	Attribution-NonCommercial-NoDerivs 3.0 United States	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/us/	*
dc.subject	soil microbiome	en_US
dc.subject	vineyards	en_US
dc.subject	soil microflora	en_US
dc.title	Extraction and purification of total DNA from soil for PCR identification of microorganism composition	en_US
dc.type	Article	en_US