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Development of the real-time PCR methodology for testing mycotoxigenic microorganisms in grape marc

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dc.contributor.author STURZA, Rodica
dc.contributor.author MITIN, Valentin
dc.contributor.author MITINA, Irina
dc.contributor.author ZGARDAN, Dan
dc.contributor.author PATRAS, Antoanela
dc.contributor.author BEHTA, Emilia
dc.date.accessioned 2021-09-28T11:37:49Z
dc.date.available 2021-09-28T11:37:49Z
dc.date.issued 2021
dc.identifier.citation STURZA, Rodica, MITIN, Valentin, MITINA, Irina. Development of the real-time PCR methodology for testing mycotoxigenic microorganisms in grape marc. In: Journal of Engineering Science. 2021, V. 28, N. 3, pp. 187-194. ISSN 2587-3474, eISSN 2587-3482. en_US
dc.identifier.uri https://doi.org/10.52326/jes.utm.2021.28(3).15
dc.identifier.uri http://repository.utm.md/handle/5014/17477
dc.description.abstract Agro-industrial waste management is an important problem of modern society as agriculture and food industry are important sources of waste. Wine production generates a considerable amount of winemaking waste (grape marc). Grape marc can be a source of natural dyes, antioxidants and could have various applications, if it is confirmed that it does not contain technogenic contaminants or unwanted microorganisms, for example, producers of mycotoxins. The paper developed the Real -Time Polymerase Chain Reaction (Real-Time PCR) methodology for testing the presence of potentially mycotoxogenic fungal species capable of producing ochratoxin A (OTA), which could be applied before grape marc processing. Based on the non-ribosomal peptide sequence of OTA, involved in ochratoxin biosynthesis, the primers have been developed for the detection of microorganisms potentially capable of producing ochratoxin A. en_US
dc.description.abstract Gestionarea deșeurilor agroindustriale prezintă o problemă importantă a societății moderne, deoarece agricultura și industria alimentară sunt surse importante de deșeuri. Producția de vin generează o cantitate considerabilă de deșeuri de vinificație (tescovină de struguri). Tescovina de struguri poate fi o sursă de coloranți naturali, antioxidanți și ar putea avea diverse aplicații, dacă se confirmă că nu conține contaminanți tehnogeni sau microorganisme nedorite, de exemplu, producători de micotoxine. Lucrarea a dezvoltat metodologia de reacție în lanț a polimerazei în timp real (PCR în timp real) pentru testarea prezenței speciilor fungice potențial micotoxogene capabile să producă ochratoxină A (OTA), care ar putea fi aplicată înainte de prelucrarea tescovinei de struguri. Pe baza secvenței peptidice non-ribozomale a OTA, implicată în biosinteza ochratoxinei, au fost dezvoltați primerii necesari pentru detectarea microorganismelor potențial capabile să producă ochratoxina A. en_US
dc.language.iso en en_US
dc.publisher Technical University of Moldova en_US
dc.relation.ispartofseries Journal of Engineering Science;2021, V. 28, N. 3
dc.rights Attribution-NonCommercial-NoDerivs 3.0 United States *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/3.0/us/ *
dc.subject wine waste en_US
dc.subject mycotoxin en_US
dc.subject grape marc en_US
dc.subject deşeuri vinicole en_US
dc.subject micotoxină en_US
dc.subject tescovină de struguri en_US
dc.title Development of the real-time PCR methodology for testing mycotoxigenic microorganisms in grape marc en_US
dc.type Article en_US


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