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Evaluation of Ultrasound Application for the Decellularization of Small Caliber Vessels

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dc.contributor.author MALCOVA, T.
dc.contributor.author NACU, V.
dc.contributor.author ROJNOVEANU, GH.
dc.contributor.author ANDRÉE, B.
dc.contributor.author HILFIKER, A.
dc.date.accessioned 2021-11-15T10:36:31Z
dc.date.available 2021-11-15T10:36:31Z
dc.date.issued 2021
dc.identifier.citation MALCOVA, T., NACU, V., ROJNOVEANU, GH. et al. Evaluation of Ultrasound Application for the Decellularization of Small Caliber Vessels. In: ICNMBE-2021: the 5th International Conference on Nanotechnologies and Biomedical Engineering, November 3-5, 2021: Program and abstract book. Chişinău, 2021, p. 90. ISBN 978-9975-72-592-7. en_US
dc.identifier.isbn 978-9975-72-592-7
dc.identifier.uri http://repository.utm.md/handle/5014/18042
dc.description Only Abstract en_US
dc.description.abstract Decellularized matrices for tissue engineering seem to be an attractive material for providing biological vascular grafts for patients with advanced peripheral arterial disease who require bypass surgery, but do not have suitable autologous small-caliber vessels (˂6 mm diameter). Currently, a variety of decellularization (DC) techniques have been proposed such as physical, chemical, and/or enzymatic methods; however, identifying an optimal protocol resulting in preservation of favorable physiochemical properties of the vascular scaffold is still elusive. The goal of the proposed study was to examine the capacity of sonication to completely decellularize small-diameter blood vessels when applied alone, to test the effect of waves’ parameters on the processing quality and matrix microarchitecture preservation, and to evaluate the possibility to reduce the time required for cell removal when ultrasound is used in combination with non-ionic detergents. Contradictory to other DC protocols reported previously, we were not able to record completely or even partially cell removal in all studied groups. Interestingly, the combination of conventional chemicals, as Triton X-100, with physical method did not result in improving the DC efficiency and did not offer tissue permeabilization and easier chemicals access towards deeper tissue layers. In addition, when high sonication power was applied, significant destruction of the vessel matrix was determined. In summary, the use of sonication had no beneficial effect on DC in this study. en_US
dc.language.iso en en_US
dc.publisher Universitatea Tehnică a Moldovei en_US
dc.rights Attribution-NonCommercial-NoDerivs 3.0 United States *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/3.0/us/ *
dc.subject ultrasound en_US
dc.subject decellularization of small caliber vessels en_US
dc.title Evaluation of Ultrasound Application for the Decellularization of Small Caliber Vessels en_US
dc.type Article en_US


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