DSpace Repository

The isolation of fibroblasts by volumetric regulation cycles

Show simple item record

dc.contributor.author JIAN, M.
dc.contributor.author COBZAC, V.
dc.contributor.author NACU, V.
dc.date.accessioned 2021-11-15T10:50:24Z
dc.date.available 2021-11-15T10:50:24Z
dc.date.issued 2021
dc.identifier.citation JIAN, M., COBZAC, V., NACU, V. The isolation of fibroblasts by volumetric regulation cycles. In: ICNMBE-2021: the 5th International Conference on Nanotechnologies and Biomedical Engineering, November 3-5, 2021: Program and abstract book. Chişinău, 2021, p. 88. ISBN 978-9975-72-592-7. en_US
dc.identifier.isbn 978-9975-72-592-7
dc.identifier.uri http://repository.utm.md/handle/5014/18044
dc.description Only Abstract. en_US
dc.description.abstract The fibroblasts are the most used cells for in vitro testing of various substances. The explant modality of fibroblast isolation is a widely used method. In order to fix the explant to the cell culture surface are used various substances or are performed various manipulations, such as applying the mechanical force or explant sticking to cell culture surface after a short period of drying. This paper presents another way of fibroblast isolation using the explant modality, which consists in manipulation with the volume of cell culture media in a well of a 12-well plate during several cycles of cell isolation. From 3 domestic rabbits, under general anesthesia, pieces of dermis were harvested and cutted to 32±8 mm3 (n=7), and placed by one per well, in a 12-well plate. For 3 days the explants were incubated in 3 ml of cell culture media to ensure the cellular multiplication on the explants. After removing the cell culture media, a small volume of medium was added to maintain the explants moist but fixed to the cell culture surface. When the explants self-attached to the cell culture surface, in the wells were poured 2 ml of cell culture media. After cellular colonies formation, the explants were transferred to another well in which the previous procedure was repeated, starting with the addition of a small volume of medium. The isolated cells from the wells, were cultured to a 80-90% confluence and subcultured in a 75cm2 flask. As a result, at 16±2 days, from the attached explants (n=6), after isolated cells subculture to a confluency of 70-80%, were obtained 2.9x106±1.6x105 cells per flask, which were identified as fibroblasts by Hematoxylin-Eosin and Masson Trichrome stainings. en_US
dc.language.iso en en_US
dc.publisher Universitatea Tehnică a Moldovei en_US
dc.rights Attribution-NonCommercial-NoDerivs 3.0 United States *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/3.0/us/ *
dc.subject fibroblasts en_US
dc.subject cells en_US
dc.subject fibroblast isolation en_US
dc.title The isolation of fibroblasts by volumetric regulation cycles en_US
dc.type Article en_US


Files in this item

The following license files are associated with this item:

This item appears in the following Collection(s)

Show simple item record

Attribution-NonCommercial-NoDerivs 3.0 United States Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 United States

Search DSpace


Advanced Search

Browse

My Account