The dynamics of potential mycotoxin producing fungi in corn samples during storage

Abstract

An alternative method for detection of mycotoxins themselves would be detecting the fungi, capable of producing mycotoxins, in food samples. In this work, we show the results of the real-time PCR analysis of four corn samples with primers, specific to the genes involved in mycotoxin synthesis and thus capable of recognizing potentially mycotoxigenic fungi. In this work, four corn kernel samples of two lines (CP148 and CP 137) harvested in 2008 and 2018, were studied. The real-time PCR analysis was done using primers specific to the genes involved in mycotoxin synthesis (fumonisin, aflatoxin, DON, T2) and Sybr Green I as a dye.