DSpace Repository

The molecular detection of brettanomyces wild yeast in raw wines

Show simple item record

dc.contributor.author ZGARDAN, Dan
dc.contributor.author MITINA, Irina
dc.contributor.author MITIN, Valentin
dc.contributor.author STURZA, Rodica
dc.contributor.author BEHTA, Emilia
dc.contributor.author RUBTOVA, Silvia
dc.date.accessioned 2022-11-19T12:59:12Z
dc.date.available 2022-11-19T12:59:12Z
dc.date.issued 2022
dc.identifier.citation ZGARDAN, Dan, MITINA, Irina, MITIN, Valentin et al. The molecular detection of brettanomyces wild yeast in raw wines. In: Modern Technologies in the Food Industry – 2022: proc. of the Intern. Conf., MTFI – 2022, 20-22 October 2022. Chişinău: Tehnica-UTM, 2022, p. 101. ISBN 978-9975-45-851-1. en_US
dc.identifier.isbn 978-9975-45-851-1
dc.identifier.uri http://repository.utm.md/handle/5014/21704
dc.description.abstract In this work, home-designed primers for detection of Brettanomyces in wine by Sybr Green I-based real-time PCR (Polymerase Chain Reaction) were developed. Primers were tested both in silico and in vitro. The primers were designed using the sequence of Brettanomyces internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene and internal transcribed spacer 2, complete sequence; and large subunit ribosomal RNA gene, partial sequence, available in GeneBank. Primer specificity was checked by aligning the primers against the sequences available in GeneBank using BLAST. Primer performance was tested using positive (wine sample infected with Brettanomyces) and negative controls. en_US
dc.language.iso en en_US
dc.publisher Universitatea Tehnică a Moldovei en_US
dc.rights Attribution-NonCommercial-NoDerivs 3.0 United States *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/3.0/us/ *
dc.subject primers en_US
dc.subject wild yeasts en_US
dc.subject raw wines en_US
dc.title The molecular detection of brettanomyces wild yeast in raw wines en_US
dc.type Article en_US


Files in this item

The following license files are associated with this item:

This item appears in the following Collection(s)

  • 2022
    20–22 October, 2022

Show simple item record

Attribution-NonCommercial-NoDerivs 3.0 United States Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 United States

Search DSpace


Advanced Search

Browse

My Account