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Effectiveness of Tissue Engineering in Obtaining the Extracellular Composite Vascularized Bone Matrix

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dc.contributor.author STOIAN, Alina
dc.contributor.author PAVLOVSCHI, Elena
dc.contributor.author CAPROŞ, Nicolae
dc.contributor.author VEREGA, Grigore
dc.contributor.author NACU, Viorel
dc.date.accessioned 2023-11-07T08:44:54Z
dc.date.available 2023-11-07T08:44:54Z
dc.date.issued 2023
dc.identifier.citation STOIAN, Alina, PAVLOVSCHI, Elena, CAPROŞ, Nicolae et al. Effectiveness of Tissue Engineering in Obtaining the Extracellular Composite Vascularized Bone Matrix. In: 6th International Conference on Nanotechnologies and Biomedical Engineering: proc. of ICNBME-2023, 20–23, 2023, Chisinau, vol. 1: Nanotechnologies and Nano-biomaterials for Applications in Medicine, 2023, p. 357-365. ISBN 978-3-031-42774-9. e-ISBN 978-3-031-42775-6. en_US
dc.identifier.isbn 978-3-031-42774-9
dc.identifier.isbn 978-3-031-42775-6
dc.identifier.uri https://doi.org/10.1007/978-3-031-42775-6_39
dc.identifier.uri http://repository.utm.md/handle/5014/24660
dc.description Acces full text - https://doi.org/10.1007/978-3-031-42775-6_39 en_US
dc.description.abstract Massive bone defects are considered to be one of the basic causes of functional disability. The gold standard, which nowadays is autologous grafting, is a perfect combination of mineralized extracellular matrix, bone marrow, and osteogenic cells. However, the available amount of such biological material is limited and the bone large defects remain a challenge. The lack of oxygen and nutrient transport actually remains the basic technical challenge in tissue engineering that limits the achievement of an effective bone allograft in the treatment of massive bone defects. The purpose of the paper is to present the results collected from the experimental study in obtaining the biocompatible extracellular composite vascularized bone matrix (vECCBM). We present a universal approach to a decellularization protocol based on the consecutive use of an isotonic solution, a chelating agent, anionic and ionic detergent as well as an enzyme solution. The effectiveness of decellularization was tested by histological examination (H&E and DAPI staining) and DNA quantification. The biocompatibility test was performed using the cultivation of the STEM cells from the bone marrow. Results: we were able to obtain a protocol for decellularization of the composite grafts, bone + vessel (soft and hard tissue) with the preservation of the vascular pedicle integrity and its connection with the bone compartment having in this way the possibility of applying anastomoses between the decellularized matrix and host. en_US
dc.language.iso en en_US
dc.publisher Springer Nature Switzerland en_US
dc.rights Attribution-NonCommercial-NoDerivs 3.0 United States *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/3.0/us/ *
dc.subject bone defects en_US
dc.subject extracellular composite bone matrix en_US
dc.subject bone allograft en_US
dc.subject tissue engineering en_US
dc.title Effectiveness of Tissue Engineering in Obtaining the Extracellular Composite Vascularized Bone Matrix en_US
dc.type Article en_US


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  • 2023
    6th International Conference on Nanotechnologies and Biomedical Engineering, September 20–23, 2023, Chisinau, Moldova

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Attribution-NonCommercial-NoDerivs 3.0 United States Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 United States

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