Methodology for creating electrophoretic passports of maize hybrids and their parental forms at the level of protein molecules

Abstract

One of the topical problems of seed production in the Republic of Moldova is the timely export of hybrid corn seeds and their quality assurance, especially varietal purity (% hybridity). The main goal of the work is to systematize the main stages of preparation and modeling of the electrophoretic passports of the reserve protein from the grain (zein) in the native corn hybrids in order to simplify the interpretation and efficiency of their use both for applied commercial purposes and in the research process.

The 65 maize hybrids and 108 maize parental lines were used as material for research according to hybrid categories and production use. Electrophoretic passporting was performed for 37 simple hybrids, 2 modified simple hybrids, 23 three-way hybrids, 2 double hybrids and one multilineal hybrid.

The initial electrophoretic spectra of zein for the parental lines of the selected hybrids were obtained by the electrophoresis method on polyacrylamide gels in acidic medium according to the national standard SM 233:2003. The obtained data were processed in the „FOREZ-2” program.