Antioxidant activity in Haematococcus pluvialis cells during the vital cycle

Abstract

The goal of this work was to determine the changes that occurred in the antioxidant status of microalga Haematococcus pluvialis during its life cycle in closed system. The method for determination of antiradicalic activity has been selected concerning its possible use for complex preparations: the ABTS˙+ radical cation scavenging assay (ABTS). The green motile stage is the most representative. Fluctuations in the antioxidant activity reflect physiological changes that occur throughout the microalga life cycle. For this stage, the antioxidant activity determined by the ABTS radical reduction correlated very strong with carotenoid content in algal biomass, R2 = 0.93 - 0.99. The green motile stage with biflagellate cells of Haematococcus pluvialis is suitable for studying variations in the antioxidant status of culture under different conditions.