Procedeul, conform invenţiei, prevede utilizarea biomasei de levuri din deşeurile
industriei de bere (30 g) care se amestecă cu 30 ml soluţie tampon fosfat de sodiu
(raport 1:1), apoi se supune autolizei la +37°C sau +45°C timp de 8 ore, cu agitare
periodică, centrifugarea, prelucrarea sedimentelor cu soluţie 1N NaOH (raport 1:5) la
temperatura de +80±5°C timp de 2 ore, apoi se efectuează centrifugarea la 3500
rot./min timp de 15 min şi supernatantele alcaline obţinute se sedimentează cu alcool
etilic 96% în volum 1:2. Procedeul permite obţinerea cu 7-16% mai multe
manoproteine comparativ cu varianta martor.
The process according to the invention includes the use of brewer's yeast biomass (30
g) which is mixed with 30 ml of sodium phosphate buffer (1:1 ratio) then subjected to
autolysis at +37°C or +45°C for 8 hours, with periodic stirring, then centrifugation and
the process of remaining the sediments with 1N NaOH solution (1:5 ratio) at +80±5°C
for 2 hours,, centrifugation at 3500 rpm. for 15 minutes, the obtained alkaline
supernatants were sedimented with 96% ethyl alcohol in a volume of 1:2. The process
allows obtaining 7-16% more mannoproteins compared to the control variant.
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 United States