Method for fungal lipases production in lab-scale fermenter

Abstract

The object of the present investigation was the fungal strain Rhizopus arrhizus CNMN FD 03 - producer of exocellular lipases. Based on the previously obtained results, the compound [Ca(L)3][Co(SCN)4], where L3 – represents the dimethyl ester of 2,6-acid pyridinedicarboxylic, was used as a potential stimulator of the lipolytic activity. Lipases production under submerged fermentation condition was carried out during 2 days in 500 mL Erlenmeyer flasks by taking 100 mL of optimized nutritive medium including the metalocomplex in a concentration of 5.0-20.0 mg/l. It was found that the optimal concentration ensuring the maximum increase (78.4% compared to the control without stimulator) of the lipolytic activity and the shortening of the technological cycle with 24 h is 10 mg/l.