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Development and Validation of SYBR Green-Based qPCR Technique of Detection and Quantification of Salmonella enterica

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dc.contributor.author STURZA, Rodica
dc.contributor.author MITIN, Valentin
dc.contributor.author MITINA, Irina
dc.contributor.author BUGA, Alexandra
dc.contributor.author ZGARDAN, Dan
dc.contributor.author BEHTA, Emilia
dc.date.accessioned 2024-05-15T08:37:35Z
dc.date.available 2024-05-15T08:37:35Z
dc.date.issued 2021
dc.identifier.citation STURZA, Rodica et al. Development and Validation of SYBR Green-Based qPCR Technique of Detection and Quantification of Salmonella enterica. In: Food and Nutrition Sciences, 2021. vol. 12, nr. 11, pp. 997-1007. ISSN 2157-944X. e-ISSN 2157-9458. en_US
dc.identifier.issn 2157-944X
dc.identifier.issn 2157-9458
dc.identifier.uri https://doi.org/10.4236/fns.2021.1211073
dc.identifier.uri http://repository.utm.md/handle/5014/27089
dc.description Acces full text - https://doi.org/10.4236/fns.2021.1211073 en_US
dc.description.abstract Salmonella enterica serovars is a leading cause of human gastroenteritis, and the incidence of salmonellosis is constantly increasing, causing millions of infections and many deaths annually. The detection of the pathogen in optimal terms is an essential factor for reducing the impact on the human body. In this work, SYBR Green I-based qPCR method of detection and quantification of Salmonella enterica was developed and validated. For detection of Salmonella enterica subsp. enterica, two pairs of primers were designed using publically available Primer-BLAST software. Primer efficiency was calculated by establishing a standard curve. The specificity, sensitivity, accuracy, and precision of PCR results were tested. Both primer pairs showed an acceptable performance, proving the developed techniques were sensitive, reliable and precise. The validated qPCR technology has a good potential to replace the traditional culture method in microbial diagnosis. en_US
dc.language.iso en en_US
dc.publisher Scientific Research Publishing Inc. en_US
dc.relation.ispartofseries Food and Nutrition Sciences;2021. vol. 12, nr. 11
dc.rights Attribution-NonCommercial-NoDerivs 3.0 United States *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/3.0/us/ *
dc.subject human gastroenteritis en_US
dc.subject Salmonella enterica en_US
dc.title Development and Validation of SYBR Green-Based qPCR Technique of Detection and Quantification of Salmonella enterica en_US
dc.type Article en_US


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