Coherent excitation of bio- molecules in three level resonance with two standing waves and its connections with Raman coherent scattering microscopy

Abstract

The coherent Raman Scattering (CRS) opens a new level of microscopy in biological systems. The CRS techniques work with the same molecular vibrations that are probed in spontaneous Raman spectroscopy. The biggest advantage, compared with spontaneous Raman spectroscopy, is the improvement in the imaging speed, which is a very attractive attribute in the biological imaging. The CRS use the nonlinear vibrational technique, which improve the readout of chemical physical information.