Analysis of plasmid deletion induced by ionizing radiation in yeast saccharomyces cerevisiae

Abstract

The objective of this study is to determine the mutagenic effects of ionizing radiation. As a model [1] we used a plasmid system for quantitative analysis of deletion formation. A can1 cyh2 cell on a YCp plasmid (with two negative markers: the CAN1 and CYH2 genes) is sensitive to canavanine and cycloheximide. This cell becomes resistant to both drugs when the plasmid has a deletion over the CAN1 and CYH2 genes.