dc.contributor.author | MITINA, Irina | |
dc.contributor.author | ZGARDAN, Dan | |
dc.contributor.author | STURZA, Rodica | |
dc.contributor.author | SCUTARU, Iurie | |
dc.date.accessioned | 2019-11-03T14:26:20Z | |
dc.date.available | 2019-11-03T14:26:20Z | |
dc.date.issued | 2019 | |
dc.identifier.citation | MITINA, Irina, ZGARDAN, Dan, STURZA, Rodica et al. The methodological aspects of using real-time polymerase chain reaction (RT-PCR) in Brettanomyces/Dekkera detection. In: Journal of Engineering Science. 2019, vol. 26(2), pp. 118-126. ISSN 2587-3474. eiSSN 2587-3482. | en_US |
dc.identifier.issn | 2587-3474 | |
dc.identifier.issn | 2587-3482 | |
dc.identifier.uri | http://repository.utm.md/handle/5014/5951 | |
dc.identifier.uri | https://www.doi.org/10.5281/zenodo.3249213 | |
dc.description.abstract | Wine spoilage could be caused by a lot of genera and species of yeasts. One of the most damaging is Brettanomyces/Dekkera which causes severe quality problems in the wine industry. Timely detection of these organisms in wine is of crucial importance for preserving wine quality. The conventional methods of diagnostics are often time-consuming and laborious. For this, molecular- ased methods of rapid testing, particularly real-time PCR are gaining increasing importance. In this work, the potential of the real-time PCR method in microbiological monitoring of wines was evaluated, and analysis process according to the PIKA WeihenstephanTM SO Detection Kit H Brettanomyces/Dekkera protocol was optimized. | en_US |
dc.description.abstract | Alterarea vinului poate fi cauzată de diferiţi agenţi patogeni, inclusiv de levuri sălbatice. Unul din genurile de levuri care cauzează probleme mari în industria vinicolă. Detecţia la timp a acestor microorganisme are un rol crucial în asigurarea unei calităţi superioare a vinurilor. Metodele moleculare de detecţie, ce se bazează pe amplificarea ADN-ului, inclusiv real-time PCR, sunt mai eficiente decât cele tradiţionale, deoarece acestea din urmă necesită mult timp și un volum mare de lucru. În această lucrare, s-a evaluat potenţialul metodei real-time PCR în monitorizarea microbiologică a vinurilor și s-a optimizat procesul de analiză în conformitate cu protocolul PIKA WeihenstephanTM SO Detection Kit H Brettanomyces/Dekkera. | ro |
dc.language.iso | en | en_US |
dc.publisher | Technical University of Moldova | en_US |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 United States | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/us/ | * |
dc.subject | red wines | en_US |
dc.subject | spoilage yeast | en_US |
dc.subject | AND | en_US |
dc.subject | deoxyribonucleic acid | en_US |
dc.subject | Brettanomyces/Dekkera | en_US |
dc.subject | PCR | en_US |
dc.subject | Polymerase chain reaction | en_US |
dc.subject | volatile phenols | en_US |
dc.subject | cycle quantification | en_US |
dc.subject | vinuri roşii | en_US |
dc.subject | drojdii de degradare | en_US |
dc.subject | en_US | |
dc.subject | acid dezoxiribonucleic | en_US |
dc.subject | fenoli volatili | en_US |
dc.subject | cuantificare cicluri | en_US |
dc.title | The methodological aspects of using real-time polymerase chain reaction (RT-PCR) in Brettanomyces/Dekkera detection | en_US |
dc.title.alternative | Aspecte metodologice ale utilizării real-time polymerase chain reaction (RT-PCR) pentru detecţia Brettanomices / Dekkera | en_US |
dc.type | Article | en_US |
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