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Interaction of Quercetin with LasR of Pseudomonas Aeruginosa: Mechanistic Insights of the Inhibition of Virulence Through Quorum Sensing

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dc.contributor.author GRABSKI, H. V.
dc.contributor.author TIRATSUYAN, S. G.
dc.date.accessioned 2020-05-29T12:40:26Z
dc.date.available 2020-05-29T12:40:26Z
dc.date.issued 2019
dc.identifier.citation GRABSKI, H. V., TIRATSUYAN, S. G. Interaction of Quercetin with LasR of Pseudomonas Aeruginosa: Mechanistic Insights of the Inhibition of Virulence Through Quorum Sensing. In: ICNMBE: International conference on Nanotechnologies and Biomedical Engineering: proc. of the 4rd intern. conf., Sept. 18-21 : Program & Abstract Book , 2019. Chişinău, 2019, p. 143. ISBN 978-9975-72-392-3. en_US
dc.identifier.isbn 978-9975-72-392-3
dc.identifier.uri https://doi.org/10.1007/978-3-030-31866-6_104
dc.identifier.uri http://repository.utm.md/handle/5014/8467
dc.description Access full text - https://doi.org/10.1007/978-3-030-31866-6_104 en_US
dc.description.abstract Pseudomonas aeruginosa is one of the most dangerous superbugs for which new antibiotics are urgently needed. This bacterium forms biofilms that increase resistance to antibiotics and host immune responses. Current therapies are not effective because of biofilms. Biofilm formation is regulated through a system called quorum sensing, which includes transcriptional regulators LasR and RhIR. These transcriptional regulators detect their own natural autoinductors. It is known that quercetin inhibits Pseudomonas aeruginosa biofilm formation, but the mechanism of action is unknown. In the present study, we tried to analyse the mode of interactions of LasR with quercetin. We show that quercetin has two binding modes. One binding mode is the interaction with ligand binding domain. This interaction is not competitive and it has been shown experimentally. The second binding mode is the interaction with the “bridge”, that involves amino acids form ligand binding domain, short linker region and DNA binding domain. This part has not been shown experimentally, because LasR protein is not soluble. In our model the hydroxyl group of ring A interacts with multiple leucines during the second binding mode. This study may offer insights on how quercetin inhibits quorum sensing circuitry by interacting with transcriptional regulator LasR. en_US
dc.language.iso en en_US
dc.publisher Tehnica UTM en_US
dc.rights Attribution-NonCommercial-NoDerivs 3.0 United States *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/3.0/us/ *
dc.subject Pseudomonas Aeruginosa en_US
dc.subject quorum sensing en_US
dc.subject flavonoids en_US
dc.subject molecular docking en_US
dc.subject molecular dynamics en_US
dc.title Interaction of Quercetin with LasR of Pseudomonas Aeruginosa: Mechanistic Insights of the Inhibition of Virulence Through Quorum Sensing en_US
dc.type Article en_US


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