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The Procedure of Bone Cells Obtaining, Culture and Identification

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dc.contributor.author JIAN, M.
dc.contributor.author COBZAC, V.
dc.contributor.author MOSTOVEI, A.
dc.contributor.author NACU, V.
dc.date.accessioned 2020-05-30T07:42:33Z
dc.date.available 2020-05-30T07:42:33Z
dc.date.issued 2019
dc.identifier.citation JIAN, M., COBZAC, V., MOSTOVEI, A. et al. The Procedure of Bone Cells Obtaining, Culture and Identification. In: ICNMBE: International conference on Nanotechnologies and Biomedical Engineering: proc. of the 4rd intern. conf., Sept. 18-21 : Program & Abstract Book , 2019. Chişinău, 2019, p. 145. ISBN 978-9975-72-392-3. en_US
dc.identifier.isbn 978-9975-72-392-3
dc.identifier.uri https://doi.org/10.1007/978-3-030-31866-6_106
dc.identifier.uri http://repository.utm.md/handle/5014/8476
dc.description Access full text - https://doi.org/10.1007/978-3-030-31866-6_106 en_US
dc.description.abstract Isolation of bone cells represent a major importance in laboratory research and clinical practice. This work was conducted on six 6±1.5 months Wistar rats from which the diaphysis of long tubular bones were harvested, followed by mechanical removal of all tissues excepting the bone tissue with further chopping. The chopped bone tissue (ChBT) was processed by two ways, each of them was followed by two methods of bone cells isolation and culture: through explant and enzymatic digestion. Obtaining of bone cells through the explant method in both ways, was more effective compared to enzymatic digestion. Also, there was no significant difference between the amounts of cells obtained between both bone processing protocols. The obtained bone cells were identified following morphological changes and Alizarin red staining after cells culture in over- confluence. en_US
dc.language.iso en en_US
dc.publisher Tehnica UTM en_US
dc.rights Attribution-NonCommercial-NoDerivs 3.0 United States *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/3.0/us/ *
dc.subject bone cells en_US
dc.subject enzymatic digestion en_US
dc.subject explant en_US
dc.title The Procedure of Bone Cells Obtaining, Culture and Identification en_US
dc.type Article en_US


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